see also on the subject:
Il fibroma uterino e l'utero fibromatoso
L'utero fibromatoso: sintomi e cura
Altre lesioni da HPV
Never neglect the loss of blood outside of your cycle, this is metrorrhagia.
You must immediately go to your gynecologist specialist and pay a visit: it could be a uterine lesion, a cancerous polyp, endometrial or cervical cancer or inflammation.
It is possible to prevent cervical cancer, and not only, through cervical
cancer screening programs implemented on a target female population, aged
between 25 and 64, by means of an ethological examination: PAP TEST.
It is called PAP TEST in honor of Papanicolau who in 1941 published the first work where he highlighted the diagnostic value of a vaginal smear to diagnose the carcinoma of the uterus.
After 1945 a great impetus
was given to the value of vaginal smear from the American Cancer Society. The
company chose as its medical director his great friend Charles Cameron. One of
the first to recognize the potential of the Pap test in reducing cervical cancer
mortality. The two met regularly to discuss the work. in short his work became a
cornerstone in the prevention of uterine cancer.
These studies were mortaled through studies carried out on the guinea pig and on the wife who daily underwent vaginal cytological samples Papanicolau has also put in place a coloring (Papanicolau coloring) that allows to properly color these smears and see the cellular alterations.
Meanwhile, the abstention from sexual relations is recommended, less than 24 hours from the withdrawal, with or without the condom, because they cause inflammatory changes (from mechanical action), alteration of the bacterial flora, possible presence of exogenous amorphous material. The presence of spermatozoa does not interfere with the evaluation of epithelial cells. In case of cervix with an excess of mucus, blood, inflammatory exudate, the cervix must be cleaned before taking it using a bottle holder to remove the excess material and then a dry gauze pad.
Do not use wadding: leave residues !;
Do not wash the cervix with a jet of water or saline: the application of saline solution alters the electrochemical balance of the cells causing artifactual aspects of swelling or breaking of the cellular structures that prevent correct microscopic evaluation. of the Pap test a small amount of cervical cells is taken with a plastic Ayre spatula (not wood) or a cytobrush and a cervical swab. Sampling of the endocervix is performed with a rotation of the Cytobrush of 90-180 °. Further rotation of the brush does not improve the representativeness of the sample and may cause bleeding.
Only the introduction of the Cytobrush, as it is covered with bristles
along its entire circumference, provides a representative sampling of the entire
endocervical canal + the rotation of the Cytobrush provides a "deeper" sampling
but must not exceed 180 ° because it is too energetic and due to bleeding. The
woman is positioned in a gynecological position, the speculum is placed and the
spatula is used to obtain a sample of cells. The spatula has a shape
complementary to the anatomy of the cervix and once inserted it is able to take
cells from the exocervice thanks to a 360 ° rotation; the tampon, on the other
hand, quite similar to those used for the pharynx, picks the cells exactly from
the endocervix, penetrating the outer uterine orifice. In the conventional pap
test, the cells are then streaked on a slide for the laboratory exam. In the
liquid phase pap test, a machine prepares a "thin layer" preparation. In the
latest generation or "thin" tests, the material is introduced into liquid
solutions, to avoid glazing fixation artefacts.
Withdrawals must be made in the various areas, even beyond the transformation region (inside) to be sure about the presence of the cells of the squamocellular junction. Usually the samples are taken in the posterior vaginal fornix and are then "crawled" on a slide that will be analyzed.
Papanicolau only gave a result that was: malignant smear, not malignant, suspected for neoplasm. Only in 1988 following the conference was a new classification proposed which is currently in use, albeit with minor modifications: it is Bethesda's classification.
The Bethesda system has the advantage of having introduced innovations that have allowed to provide indications (there are / there are no malignant cells) in a real diagnostic finding that tells us what kind of injury there is in that particular smear in that particular moment. It has also introduced new terminologies, such as squamous intraepithelial lesion (SIL) which is nothing but the cytological equivalent of CIN.
The term introduced is therefore a synonym for dysplasia and has the
advantage of dividing lesions into two groups: high and low-grade (L-SIL and
H-SIL, low-SIL and high-SIL), because the ability to distinguish lesions in two
groups from a greater reliability than a classification in several groups: if
you have to classify anything less "containers" there are and fewer mistakes
there are! It also has a clinical value because low-grade lesions can be treated
one way, high-grade lesions in another: between the L-SILs, the condylomatous
lesions and the CINI are included, among the H-SIL are the CIN2 and the
I have told you that most CIN1 regresses, so for the L-SIL a "non-invasive"
surveillance will be sufficient while for the H-SIL (cin2 3) it is necessary to
deepen the type of surveillance with colposcopy, biopsy samples etc. ..
Another introduction was that of ASCUS, a container where doubtful cases are put in: we keep in mind that we are always analyzing cells that have been broken and not a histological preparation; the detached cells may have alterations that may be dysplastic or other alterations (inflammation etc.) so if there is no certainty these cells are classified in this standby condition that requires further evaluation in different ways (which we will see later).
Obviously the levy must take into account the cycle: it must be done around the tenth-twelfth day of the cycle, then away from menstruation because the blood could cause covers, masks.
The report may be negative for intraepithelial lesions or malignancy but positive for infection; this infection must be indicated and is at the base of the cervical inflammation of the cervix.
Mushrooms (for example, Candida)
Bacteria (for example, Gardnerella)
Virus (for example, Papilloma Virus or HPV)
Trichomonas, a protozoan that causes inflammation
other types of intra-epithelial lesions.
coilocyte is a squamous epithelial cell that has undergone a series of
structural changes, which occur as a result of an infection of the cell by the
human papilloma virus.
The coilocytes may have the following nuclear alterations:
- Nuclear enlargement
- granules of chromatin-citrolols
- variations in the size and shape of the core
Nuclear alterations are accompanied by cytoplasmic "halos", formed, at an ultrastructural level, by perinuclear vacuoles, a cytopathic change caused by E5, a protein encoded by HPV, which is localized in the membranes of the endoplasmic reticulum. Nuclear alterations with the associated perinuclear halo are called "coilocytic atypia".
there are cells that exhibit alterations that could be interpreted as high-grade
lesions, so skip that phase of CIN1; then we have L-SIL, H-SIL and squamous
carcinoma of the cervix. Among the glandular we have atypical cells, neoplastic
cells and carcinoma. This type of screening based on the recall of women,
between 25 and 64 years, every 2-3 years has led today to encouraging results:
in the last 30 years in Italy the carcinoma of the cervix has been reduced by
2/3. The PAP test, or the evaluation of the smear, represents the most powerful
screening system currently existing in oncology, this is because it is based on
two facts: women are called periodically in the critical period of onset and
accuracy of the test. From 20 / 100,000 cases to 7 / 100,000 cases in the last
Let's go back to the Coilocyte we saw earlier: it represents the alteration determined by the HPV virus, they are atypical cells that have a perinuclear halo.
few years ago, and even now in some parts, the HPV TEST is proposed in the
triage of ASCUS lesions. We said that if we have an ASCUS we do not know what it
is -> let's do the HPV TEST -> If the test is positive it means that these
lesions are dysplastic and we do the colposcopy; if the test is negative, it
must be repeated within 6 months.
Currently in Italy the HPV TEST is foreseen in three conditions: triage of the borderline ASCUS cytology in the primary screening of experimental studies in the case of treated pts (to see if the virus persists or not)
In the triage of borderline cytology the PAP test has a variable sensitivity, not high, while the HPV TEST has a very high sensitivity: then this test is important not because there is an infection but if it DOES NOT 'the infection or who does not has the infection can feel comfortable (has negative predictive value); who has the infection does not mean anything!
It has recently been proposed to overturn the situation: first use the HPV TEST, which is more sensitive and less specific, to select all infected people and then this group is subjected to triage with cytology, which is more specific. This is a guideline now used in America, in Italy instead we do it experimentally only in two regions but has not yet been extended to the whole nation.
HPV is contracted by women in 80% of the female population during their lifetime; 46% have a high probability of contracting the virus three years after their first sexual relationship, even though they only had one relationship. So the concept "+ sexual intercourse = + possibility of contracting the virus" is true but up to a certain point! In fact, the virus can, albeit less, be contracted even with contaminated objects that come into contact.
There is a quadratic anti-HPV vaccine (anti 6-11-16-18) also proposed free from the Sicilian region to adolescents to prevent significant damage; remember, however, that the vaccine only covers 1/3 of the viruses circulating.
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lesioni tumorali e pap test